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Arun Kumar, S.
- The GC MS Study of one Ayurvedic Medicine, Vasakadyaristam
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Authors
Muttevi Hyagreva Kumar
1,
K. Prabhu
2,
Mudiganti Ram Krishna Rao
3,
R. Lakshmi Sundaram
4,
Sampad Shil
5,
S. Arun Kumar
5
Affiliations
1 Sree Balaji Medical College and Hospital, Bharath University, Chrompet, Chennai - 600092, IN
2 Department of Anatomy, Sree Balaji Medical College and Hospital, Bharath University, Chrompet, Chennai - 600092, IN
3 Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Chennai-600073, IN
4 Central Research Facility, Sri Ramachandra Medical College and Research Institute, Purur, Chennai - 600116, IN
5 Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Chennai- 600073, IN
1 Sree Balaji Medical College and Hospital, Bharath University, Chrompet, Chennai - 600092, IN
2 Department of Anatomy, Sree Balaji Medical College and Hospital, Bharath University, Chrompet, Chennai - 600092, IN
3 Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Chennai-600073, IN
4 Central Research Facility, Sri Ramachandra Medical College and Research Institute, Purur, Chennai - 600116, IN
5 Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Chennai- 600073, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 2 (2019), Pagination: 569-573Abstract
The present study deals with the GC MS analysis of one Ayurvedic prepaation, Vasakadyarishtam, which is a mucolytic medicine used for the treatment of cough, cold, chest congestion asthma and related diseases like bronchitis, inflammation and hemoptysis. The most important constituent of this medicine is Vasaka or Adhatoda vesika plant, which is known for its anti-cough and cold usage in Ayurveda. This study was to understand the main biomolecules present in this medicine by GC MS analysis. The results indicted the presence of some important molecules such as Eucalyptol, Phenylethyl alcohol, Camphor, Ethyl hydrogen succinate, Succinic acid, cyclobutyl ethyl ester, 3-Cyclohexen-1-ol,4-methyl-(1-methylethyl)-(R)-,. alpha.-Terineol, 5- Hydroxymethyl furfural, p-Anisic acid,4-nitrophenyl ester, 2-Propen-1-0l,3-phenyl, Benzeneethanol, 4-hydroxy- Propiophenone, 2‘-(trimethylsioxy)-, Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester and Octadecanoic acid and 2,3 –dyhydroxypropyl ester. These molecules are known for their various medicinal properties which indicate their roles in this formulation.Keywords
Vasakadyarishtam, Adhatoda vesika, Eucalyptol, Phenylethyl Alcohol, Camphor, Ethyl Hydrogen Succinate, Alpha.-Terineol.References
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- Evaluation of Genetic Diversity among Ocimum sanctum
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Authors
Affiliations
1 Department of Industrial Biotechnology, BIHER, Selaiyur, Chennai-600073, IN
1 Department of Industrial Biotechnology, BIHER, Selaiyur, Chennai-600073, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 6 (2019), Pagination: 2889-2894Abstract
In this study the evaluation of genetic diversity among Ocimum sanctum was undertaken. The method used to find variation among Ocimum sanctum was restriction fragment length polymorphism excluding blot hybridization. The Ocimum sanctum plants were taken from five different regions of Tamil Nadu - Chennai, Karaikudi, Kancheepuram, Vellore, Tanjavur and Puducherry. Along with whole plants the soil and water samples in the regions of the plant was also collected. DNA was isolated using CTAB protocol with ammonium acetate and ethanol precipitation. Then the DNA was quantified and restriction digested with EcoR1 and HindIII (double digested). Double digested Lambda DNA (EcoRI and Hind III) was used as marker and the samples along with marker were electrophoresed in 0.8% agarose gel at 80V and the bands were observed in UV trans illuminator. Based on observation of bands the plants from Karaikudi, Tanjavur, Kancheepuram and Puducherry may have been descended from Chennai plants and Vellore plant may have been descended from Karaikudi plant. There was sufficient variation to predict the migration pattern of the plant. And variation among water-hardness and pH and soil-N, P, K content and pH was also diverse. The measured soil and water characteristics may be few of the reasons for the variation among Ocimum sanctum around Tamil Nadu and Puducherry.Keywords
Ocimum sanctum, CTAB, DNA Extraction, Restriction Digestion, Nutrient of Soil And Hardness.References
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